Development of an in vitro model of kidney collecting duct using mCCDcl1 cells

Development of an in vitro model of kidney collecting duct using mCCDcl1 cells

mCCDcl1, a mouse renal cell line, has been shown to be a good model for the study of collecting duct physiology, and cell differentiation. mCCDcl1 cells are cultured on a 3D-printed porous scaffold for the development of a 3D in vitro model of collecting duct.

In the mammalian kidney, the collecting duct plays an important role in the regulation of several key renal functions such as body fluid volume, acidity, and salt reabsorption. The collecting duct is composed of two cell types: principal cells and intercalated cells. mCCDcl1 is a cell line from mouse collecting ducts that showed functions of principal cells, and used as such in previous studies. In this project, we showed that mCCDcl1 present characteristics of both principal and intercalated cells, as well as evidence for plasticity between the two cell types. A large part of the population showed characteristics of both types in the same cells, and these characteristics were transmitted to new populations through a single cell. To get closer to optimal cell culture conditions, a 3D model of collecting duct was designed and developed using 3D-printing of porous polymers. Small porous tubes were printed and used as a cell culture scaffold. mCCDcl1 cells showed improved characteristics compared to classic culture conditions, and can be used as a model for the study of collecting duct physiology and development.

Principal Investigator, Other investigators:

John Mullins (PI)

Adrienne Assmus

Other: Linda Mullins, Morag Mansley, Colin Sherborne, John Haycock